I was thrown off balance today when my PCRs worked. My plan for the afternoon was to start from scratch with new dNTPs, frsh DNA preparation etc so I had to quickly figure out what to do next. The answer turned out to be more PCRs as i need more of the product to do the digestions afterwards.
This puts the KO method ever-so-slightly ahead of the P-element excision. Then again it is possible that I could cross my isogenised (in bred) P-element flies to the transposase next Friday.