I think it's a good thing that scientists are being a bit more open about why they do things in experiments the way they do. It makes us seem a bit more human and fallible.
If I were to admit to some they'd be along these lines and I suspect they'd be true for a lot of biologists.
- Flies are kept at 25 degrees because then I don't need to come in at weekends.
- Secondary antibodies are left on the sample for 2 hours because sometimes I like a long lunch or a quick drink in the evening.
- I worked on a project because the wings looked fat (short and round) and not because they looked like Fat (protein knock down). Luckily it actually did interact with Fat.
- I do X method because that's how it worked - I have no idea why it worked and honestly don't care that much.
- I use euparal fixing method because you can order it, rather than make it and it smells nice.
- When I try to summarise a method with the phrase "basically" you can guarantee there's nothing basic about it.
- sometimes I find an interesting result by accident and then have to concoct a legend as to why this was what I was expecting to find all along.
- when a method says "72 hours" later I tend to do 10 or 14 as staying at work until 9pm or starting at 7am is too painful.